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#18952 Dec 02 2012 at 2:12 AM Rating: Good
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He's at least 12 so its ok
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#18953 Dec 02 2012 at 3:27 AM Rating: Good
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More drinking, less contemplating age.

Snow has finally arrived here as well and the weather guys are saying it'll stay. Unlike smunks, I can find my gloves. Unfortunately, there's a big hole in one of them. Smiley: crymore It's going to be cold getting to work tomorrow.
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#18954 Dec 02 2012 at 3:33 AM Rating: Decent
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We don't get snow very often at all except in the mountains.

I kind of wish we got more. The city shuts down when we get an inch or two (combination of crazy hilly roads and two plows for the whole metro area).
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#18955 Dec 02 2012 at 5:14 AM Rating: Good
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I hate you all for getting snow while all I'm getting is rain. And rain and rain and did I mention the rain yet?
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Theophany wrote:
YOU'RE AN ELITIST @#%^ AETHIEN, NO WONDER YOU HAVE NO FRIENDS AND PEOPLE HATE YOU.
someproteinguy wrote:
Aethien you take more terrible pictures than a Japanese tourist.
Astarin wrote:
One day, Maz, you'll learn not to click on anything Aeth links.
#18956 Dec 02 2012 at 6:16 AM Rating: Decent
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His Excellency Aethien wrote:
I hate you all for getting snow while all I'm getting is rain. And rain and rain and did I mention the rain yet?

I like rain. But rain in Seattle means decently cold, and boot/sweater weather, AKA my favorite time of the year.
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#18957 Dec 02 2012 at 2:05 PM Rating: Good
PigtailsOfDoom wrote:
Naughty Fishy! You're too young to drink. Smiley: tongue


Yeah, and oh god was I miserable last night, too.

Fortunately I was with friends who were far more sober than me and took care of me while I cleaned out my stomach.
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#18958 Dec 02 2012 at 3:03 PM Rating: Good
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Can't hold yer liquor, eh, grasshopper?

Not that I'm one to talk. I can hold my liquor as long as it comes in controlled doses. The thing where you drink a bottle of vodka and six beers in 20 minutes... it leads to me passing out and waking up hours later in random places, usually with someone there to tell me about all the stupid stuff I did while I was out.

Now I only drink in moderation. Smiley: schooled

I actually have this t-shirt.
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#18960 Dec 02 2012 at 5:06 PM Rating: Good
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Did something just get nuked? Shows one new reply, but the preview is blank and my post is the last post. Smiley: dubious
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#18961 Dec 02 2012 at 5:16 PM Rating: Good
I think something did.

And I at least kept it together better than some of the other people there, believe me.
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idiggory, King of Bards wrote:
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#18962 Dec 02 2012 at 6:20 PM Rating: Good
If it makes you feel better Aeth, i'm not getting any rain.

I have been sick exactly twice from drinking. Once was when I was 26, and my bf did it to me on purpose because I kept bragging about how I'd never gotten sick or had a hangover before. He made me a drink with Jose Cuervo tequilia and Hypnotiq, and then made me another with Don Julio tequilia and Hypnotiq. Not fun. The other time was a few weeks ago when I had three drinks on an empty stomach and then decided that jalepeno nachos would be a good cure for that. I had two rum and cokes and one screwdriver I think.
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#18963 Dec 02 2012 at 6:26 PM Rating: Good
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I threw up a month ago from drinking way too much whiskey after drinking many beers.

The time before that was probably about 6 years ago. I expect to easily go another 6+ years before it happens again.
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idiggory wrote:
Drinking at home. But I could probably stand to get laid.
#18964 Dec 03 2012 at 3:10 AM Rating: Good
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Overlord Theophany wrote:
His Excellency Aethien wrote:
I hate you all for getting snow while all I'm getting is rain. And rain and rain and did I mention the rain yet?

I like rain. But rain in Seattle means decently cold, and boot/sweater weather, AKA my favorite time of the year.
It's because you 'mericuns don't cycle everywhere. Rain is fun when you're inside but not fun at all when it means you get really @#%^ing wet.

Also, snow now. Yay snow. It's still turning to filthy brown mush as soon as it hits the ground but it looks pretty while falling.

edit: 10 minutes of bliss and wonderful music:


Edited, Dec 3rd 2012 11:02am by Aethien
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Theophany wrote:
YOU'RE AN ELITIST @#%^ AETHIEN, NO WONDER YOU HAVE NO FRIENDS AND PEOPLE HATE YOU.
someproteinguy wrote:
Aethien you take more terrible pictures than a Japanese tourist.
Astarin wrote:
One day, Maz, you'll learn not to click on anything Aeth links.
#18965 Dec 03 2012 at 9:40 AM Rating: Excellent
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Mazra wrote:
More drinking, less contemplating age.

There's a dirty joke in there somewhere, but its too early in the morning to figure out what it is.

His Excellency Aethien wrote:
Overlord Theophany wrote:
His Excellency Aethien wrote:
I hate you all for getting snow while all I'm getting is rain. And rain and rain and did I mention the rain yet?

I like rain. But rain in Seattle means decently cold, and boot/sweater weather, AKA my favorite time of the year.
It's because you 'mericuns don't cycle everywhere. Rain is fun when you're inside but not fun at all when it means you get really @#%^ing wet.

Bah whatever, so of us commuted by bike for years. Snow and ice don't make for a very pleasant ride, especially when you add in the dark, the hills, and the fact no one here seems to be able to drive in the stuff. Not that your Geo Metro really stands a chance on a sheet of ice. Smiley: rolleyes
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#18966 Dec 03 2012 at 12:38 PM Rating: Good
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At work, two more hours to go. Bored. Who's in for a beer after work?


#18967 Dec 03 2012 at 1:31 PM Rating: Decent
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His Excellency Aethien wrote:
Overlord Theophany wrote:
His Excellency Aethien wrote:
I hate you all for getting snow while all I'm getting is rain. And rain and rain and did I mention the rain yet?

I like rain. But rain in Seattle means decently cold, and boot/sweater weather, AKA my favorite time of the year.
It's because you 'mericuns don't cycle everywhere. Rain is fun when you're inside but not fun at all when it means you get really @#%^ing wet.

I run in it (and I run pretty much every day). I play lacrosse in it. Still love it.

Actually, rainy lacrosse games and rainy runs are better than my normal runs.
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#18968 Dec 03 2012 at 4:11 PM Rating: Good
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In that case you're just batsh*tcrazy.
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Theophany wrote:
YOU'RE AN ELITIST @#%^ AETHIEN, NO WONDER YOU HAVE NO FRIENDS AND PEOPLE HATE YOU.
someproteinguy wrote:
Aethien you take more terrible pictures than a Japanese tourist.
Astarin wrote:
One day, Maz, you'll learn not to click on anything Aeth links.
#18969 Dec 03 2012 at 4:48 PM Rating: Excellent
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It's not really so bad. It's not like it's pouring most days here, and a misty-drizzle is usually not going to soak all the way through during a 30min jog. If you're wearing some of those thin gloves maybe your hands will be a bit frigid, but it was pretty bearable overall.

Also, some good memories of soccer in the rain. It's just tons more fun that way. Smiley: lol
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#18970 Dec 03 2012 at 5:45 PM Rating: Decent
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Meh, lacrosse I generally wear Under Armour long sleeve/leggings combination. Running, I never get cold enough to need more than shorts and a windbreaker.

Water wicking/proof gear is well worth their weight in gold. I don't wear them when I'm out and about normally (as I can just use an umbrella), but for sports and outdoor activities, definitely worth the price.
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#18971 Dec 03 2012 at 5:53 PM Rating: Good
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Whenever I'm out in the rain, I just think back to my military service.

One week in particular stands out: It was late November and we were out on a four-day drill (Monday morning to Thursday evening). It rained non-stop during the day and then the temperature dipped below the freezing point during the night. I remember one exceptionally cold night where I had been on guard duty in our camp for two hours, which involved lying completely still in the wet grass while staring into darkness with night vision goggles on, listening and looking for movement. My body was completely sore all over because of the shivering and my face was hurting because of my teeth clattering. When my replacement arrived and I got up, my clothes crunched like ice breaking on a lake. It was so loud the enemy team's scouts heard it and opened fire.

You feel kinda tired of life when a sergeant is berating you for alerting the enemy team with the sound of your frozen clothes, the result of you doing an outstanding job at not @#%^ing moving while on guard duty. Those four days were Hell on Earth and I'm surprised I kept it together that long, but they gave me perspective. Who gives a sh*t about 30 minutes in a drizzle or a downpour compared to 72 hours in alternating downpour and frost? 30 minutes is nothing, and once those 30 minutes are up, you get to spoil yourself with hot chocolate and blankets. You don't get yelled at and then have to climb into a wet, frozen sleeping bag in your wet, frozen clothes and sleep for three hours before having to get up again for some stupid march.

The only rainy situation I can't stand is when it's windy at the same time. The chill factor it causes brings back memories of that night in November when I was ready to lie down and die.
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#18972 Dec 03 2012 at 6:19 PM Rating: Good
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someproteinguy wrote:
It's not really so bad. It's not like it's pouring most days here, and a misty-drizzle is usually not going to soak all the way through during a 30min jog.
I'm talking proper rain here, the kind that'll make your jeans soak up enough water to fill a bathtub in about 5 minutes.
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Theophany wrote:
YOU'RE AN ELITIST @#%^ AETHIEN, NO WONDER YOU HAVE NO FRIENDS AND PEOPLE HATE YOU.
someproteinguy wrote:
Aethien you take more terrible pictures than a Japanese tourist.
Astarin wrote:
One day, Maz, you'll learn not to click on anything Aeth links.
#18973 Dec 03 2012 at 7:58 PM Rating: Good
Dear diary,

Today I learned that Maz used to be a badass.
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#18974 Dec 03 2012 at 11:15 PM Rating: Good
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His Excellency Aethien wrote:
someproteinguy wrote:
It's not really so bad. It's not like it's pouring most days here, and a misty-drizzle is usually not going to soak all the way through during a 30min jog.
I'm talking proper rain here, the kind that'll make your jeans soak up enough water to fill a bathtub in about 5 minutes.


A few years ago, 06, it rained non-stop for 40 days and 40 nights out here. Kind of sucked after the first 20 days.
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idiggory wrote:
Drinking at home. But I could probably stand to get laid.
#18975 Dec 04 2012 at 1:31 AM Rating: Good
Did you end up having to board a huge boat?
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idiggory, King of Bards wrote:
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#18976 Dec 04 2012 at 1:44 AM Rating: Good
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IDrownFish of the Seven Seas wrote:
Did you end up having to board a huge boat?


I can swim so it was ok.

The stupids went out on a boat and eventually died of starvation.
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idiggory wrote:
Drinking at home. But I could probably stand to get laid.
#18977 Dec 04 2012 at 1:49 AM Rating: Good
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Can't really remember a case where it would have rained an awafl lot in here. Except a cople months back, when it rained every day for like a week.
Then again snow is a different story, I don't know what's up with that.
#18978 Dec 04 2012 at 2:58 AM Rating: Decent
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His Excellency Aethien wrote:
someproteinguy wrote:
It's not really so bad. It's not like it's pouring most days here, and a misty-drizzle is usually not going to soak all the way through during a 30min jog.
I'm talking proper rain here, the kind that'll make your jeans soak up enough water to fill a bathtub in about 5 minutes.

Seattle doesn't really get rain like that. It rains a lot, but never hard.

You're just living in the wrong place. Smiley: schooled
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#18979 Dec 04 2012 at 3:48 AM Rating: Good
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IDrownFish of the Seven Seas wrote:
Dear diary,

Today I learned that Maz used to be a badass.
The army is a crazy place...

My great granddad was a general something or other (I have no clue of the details), my granddad made it to captain, major, something relatively high up anyway, my dad made it to corporal and I wouldn't touch the army with a ten foot pole. I very much approve of this progression, I wouldn't have lasted more than a week in the army.
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Theophany wrote:
YOU'RE AN ELITIST @#%^ AETHIEN, NO WONDER YOU HAVE NO FRIENDS AND PEOPLE HATE YOU.
someproteinguy wrote:
Aethien you take more terrible pictures than a Japanese tourist.
Astarin wrote:
One day, Maz, you'll learn not to click on anything Aeth links.
#18980 Dec 04 2012 at 8:26 AM Rating: Good
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His Excellency Aethien wrote:
The army is a crazy place ...
Where isn't?
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#18981 Dec 04 2012 at 9:40 AM Rating: Excellent
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Horsemouth wrote:
His Excellency Aethien wrote:
someproteinguy wrote:
It's not really so bad. It's not like it's pouring most days here, and a misty-drizzle is usually not going to soak all the way through during a 30min jog.
I'm talking proper rain here, the kind that'll make your jeans soak up enough water to fill a bathtub in about 5 minutes.


A few years ago, 06, it rained non-stop for 40 days and 40 nights out here. Kind of sucked after the first 20 days.


Worst rain I've even been in was on Kahekili Hwy. Think we were going like 10 mph, the wipers were on full and I couldn't see through the windshield. I decided I wouldn't want to live in a place where that was normal.
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#18982 Dec 04 2012 at 4:11 PM Rating: Good
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I love rain in the summer. The change in atmospheric pressure just before the rain hits, where the heat and humidity is replaced by a crisp and slightly chilly wind and then complete silence. Even better if it's a thunderstorm! You can almost feel the electricity in the air. Smiley: yippee
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#18983 Dec 04 2012 at 10:21 PM Rating: Good
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someproteinguy wrote:
Horsemouth wrote:
His Excellency Aethien wrote:
someproteinguy wrote:
It's not really so bad. It's not like it's pouring most days here, and a misty-drizzle is usually not going to soak all the way through during a 30min jog.
I'm talking proper rain here, the kind that'll make your jeans soak up enough water to fill a bathtub in about 5 minutes.


A few years ago, 06, it rained non-stop for 40 days and 40 nights out here. Kind of sucked after the first 20 days.


Worst rain I've even been in was on Kahekili Hwy. Think we were going like 10 mph, the wipers were on full and I couldn't see through the windshield. I decided I wouldn't want to live in a place where that was normal.


I've told you many times, Kaneohe is a terrible place.
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Drinking at home. But I could probably stand to get laid.
#18984 Dec 05 2012 at 9:32 AM Rating: Excellent
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Horsemouth wrote:

I've told you many times, Kaneohe is a terrible place.

Sometimes I need to be reminded repeatedly of these things; I blame old age.
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#18985 Dec 05 2012 at 9:52 AM Rating: Good
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HAHA, YER OLD!
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#18986 Dec 05 2012 at 10:21 AM Rating: Good
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Age is a state of mind, and yours is Montana.
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#18987 Dec 05 2012 at 1:07 PM Rating: Excellent
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I like Montana. I mean Glacier National Park used to be pretty before all the glaciers melted. There's probably an analogy there...

Also:

I HATE REGULAR EXPRESSIONS! Smiley: mad
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#18988 Dec 05 2012 at 1:38 PM Rating: Excellent
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There done! My first actual program that does something useful around the lab. Smiley: grin

It's ugly, but it works... Smiley: tongue

import Tkinter 
import tkFileDialog 
import re 
 
root = Tkinter.Tk() 
root.withdraw() 
 
openfile = tkFileDialog.askopenfilename(parent=root,title='Open file') 
fastafile = open(openfile) 
line = fastafile.readline(0) 
accessions = '' 
sequences = '' 
 
for line in fastafile: 
    if line.startswith('>'): 
        stripped1 = line.strip('\n') 
        accessions = accessions + stripped1 
        sequences = sequences + '\n' 
    else: 
        stripped2 = line.strip('\n') 
        sequences = sequences + stripped2 
         
accessions2 = accessions 
accessions3 = accessions.replace(' %s>', ' REVERSED >') 
accessions3 = re.sub('( .*?>)', ' REVERSED >', accessions) 
accessionlist = accessions2.split('>') 
sequenceslist = sequences.split('\n') 
del sequenceslist[0] 
del accessionlist[0] 
reversedsequences = sequences[::-1] 
reversedaccessions = accessions3.replace('>', '>REV_') 
reversedsequenceslist = reversedsequences.split('\n') 
reversedaccessionlist = reversedaccessions.split('>') 
reversedaccessionlist.reverse() 
del reversedaccessionlist[-1] 
del reversedsequenceslist[-1] 
 
output = open('newdatabase.fasta', 'w') 
for x,y in zip(accessionlist, sequenceslist): 
    print >>output, '>' + '%s\n%s' %(x, y) 
for x,y in zip(reversedaccessionlist, reversedsequenceslist): 
    print >>output, '>' + '%s\n%s'% (x, y) 
output.close()


Smiley: yippee
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#18989 Dec 05 2012 at 5:00 PM Rating: Good
I'm too lazy to dig through the thread again. That's Python, right? Other than that, no clue what the hell that does. I mean, takes a file and reads... something.
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#18990 Dec 05 2012 at 5:29 PM Rating: Excellent
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IDrownFish of the Seven Seas wrote:
That's Python, right?


Smiley: nod

IDrownFish of the Seven Seas wrote:
Other than that, no clue what the hell that does.


Next thing I learn: how to document my code. Smiley: grin

IDrownFish of the Seven Seas wrote:
I mean, takes a file and reads... something.


You give it this:

>CONT_003 VATVSLPR-like Promega trypsin artifact 3 (824.5) xxxVSLPR (Trypa3|PromTArt3).
PGVVSLPR
>CONT_004 LSSPATLNSR-like Promega trypsin artifact 4 (1071.5) xxxPATLNSR (Trypa4|PromTArt4).
MNTLPLLAAK

It returns this:

>CONT_003 VATVSLPR-like Promega trypsin artifact 3 (824.5) xxxVSLPR (Trypa3|PromTArt3).
PGVVSLPR
>CONT_004 LSSPATLNSR-like Promega trypsin artifact 4 (1071.5) xxxPATLNSR (Trypa4|PromTArt4).
MNTLPLLAAK
>REV_CONT_004 REVERSED
KAALLPLTNM
>REV_CONT_003 REVERSED
RPLSVVGP

And that's about all I can do at this point... Smiley: lol
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#18991 Dec 05 2012 at 7:00 PM Rating: Good
Screenshot.
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#18992 Dec 06 2012 at 9:04 AM Rating: Good
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I think that's code for Orange Soda.
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#18993 Dec 06 2012 at 9:42 AM Rating: Good
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Not for boobs? I bet it's for boobs.

Edited, Dec 6th 2012 4:43pm by Aethien
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Theophany wrote:
YOU'RE AN ELITIST @#%^ AETHIEN, NO WONDER YOU HAVE NO FRIENDS AND PEOPLE HATE YOU.
someproteinguy wrote:
Aethien you take more terrible pictures than a Japanese tourist.
Astarin wrote:
One day, Maz, you'll learn not to click on anything Aeth links.
#18994 Dec 06 2012 at 9:48 AM Rating: Excellent
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Everything should be for boobs.
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#18995 Dec 06 2012 at 10:29 AM Rating: Good
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Especially ones like Salma Hayek's.
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Theophany wrote:
YOU'RE AN ELITIST @#%^ AETHIEN, NO WONDER YOU HAVE NO FRIENDS AND PEOPLE HATE YOU.
someproteinguy wrote:
Aethien you take more terrible pictures than a Japanese tourist.
Astarin wrote:
One day, Maz, you'll learn not to click on anything Aeth links.
#18996 Dec 06 2012 at 10:38 AM Rating: Good
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Legs > Breasts

Prove me wrong.
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#18997 Dec 06 2012 at 11:11 AM Rating: Good
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whoops

Edited, Dec 6th 2012 6:12pm by Aethien
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Theophany wrote:
YOU'RE AN ELITIST @#%^ AETHIEN, NO WONDER YOU HAVE NO FRIENDS AND PEOPLE HATE YOU.
someproteinguy wrote:
Aethien you take more terrible pictures than a Japanese tourist.
Astarin wrote:
One day, Maz, you'll learn not to click on anything Aeth links.
#18998 Dec 06 2012 at 11:12 AM Rating: Good
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lolgaxe wrote:
Like this?
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Theophany wrote:
YOU'RE AN ELITIST @#%^ AETHIEN, NO WONDER YOU HAVE NO FRIENDS AND PEOPLE HATE YOU.
someproteinguy wrote:
Aethien you take more terrible pictures than a Japanese tourist.
Astarin wrote:
One day, Maz, you'll learn not to click on anything Aeth links.
#18999 Dec 06 2012 at 11:47 AM Rating: Good
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His Excellency Aethien wrote:
lolgaxe wrote:
Like this?
Like this? Smiley: dubious
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#19000 Dec 06 2012 at 12:15 PM Rating: Good
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Whoever did the photoshopping in that picture did a terrible job.
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Theophany wrote:
YOU'RE AN ELITIST @#%^ AETHIEN, NO WONDER YOU HAVE NO FRIENDS AND PEOPLE HATE YOU.
someproteinguy wrote:
Aethien you take more terrible pictures than a Japanese tourist.
Astarin wrote:
One day, Maz, you'll learn not to click on anything Aeth links.
#19001 Dec 06 2012 at 12:39 PM Rating: Good
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